The NanoInflammation Team focuses on studying the interactions of nanomaterials with the immune system and associated diseases, using in vitro and in vivo models. We investigate the consequences of nanomaterial interactions with different biological systems (pulmonary, central nervous, cardiovascular, lymphatic, and mononuclear phagocytic systems) at various scales (time, dose, duration) in order to reveal their safety limitations in the context or not of biomedical application.
Our objectives are not only to identify the physicochemical properties causing adverse outcomes, but also to find the biological mechanisms triggered by the nanomaterial-biological system interaction. By correlation of specific biological responses to the nanomaterial physicochemical properties, and revealing what is safe and what causes harm, the knowledge we are creating helps the production of nanomaterials that are safer-by-design for the patient or the end-user in the intended application.
To address these questions, we use state-of-the-art techniques as well as contribute to the development of new technical modalities to study the nanomaterial-biology interface. We work at all scale levels (from molecules up to full organism), using either specific biomarkers in an adverse outcome pathway approach, or high-content methods to decipher the complexity of the nano/bio interface. We engage with stakeholders such as nanomaterial production industries (chemical or pharmaceutical companies), regulators or standards institutions, in order to make our experimental work as realistic and exposure-driven as possible.
Health impact of engineered nanomaterials
We investigate the impact of (nano)materials on human health in an occupational, end-users or biomedical context. Using different routes of exposure, we study the impact on primary and secondary organs, focusing primarily on the innate and adaptive immune responses. Current investigations are assessing the impact of carbon based materials, 2D materials and micro- /nano- plastics after single or repeated exposure in various in vitro and in vivo models.
Correlative microscopy for toxicological investigations
Using the innate chemical signature of nanomaterials, we perform correlative microscopy to understand the impact of nanomaterials in respect to their spatial location in tissue and cells. In the past we used X ray fluorescence microscopy and light microscopy; in more recent works, we correlated Raman imaging with immunofluorescence imaging.
Dr. Cyril Bussy
LecturerNanoInflammation Team Leader
Dr. Thomas Loret
Mr. Alex Fordham
Journal of Hazardous Materials, 2022, in press
Hazard assessment of abraded thermoplastic composites reinforced with reduced graphene oxide
Advanced Science, 2022, 9, 2104559
Innate but not adaptive immunity regulates lung recovery from chronic exposure to graphene oxide nanosheets
Nanoscale Advances, 2021, 3 (14), 4166-4185
Dynamic interactions and intracellular fate of label-free, thin graphene oxide sheets within mammalian cells: Role of lateral sheet size
Langmuir, 2021, 37 (2), 867-873
Adsorption of P103 nanoaggregates on graphene oxide nanosheets: Role of electrostatic forces in improving nanosheet dispersion
Advanced Therapeutics, 2021, 4 (1), 2000109
Deep tissue translocation of graphene oxide sheets in human glioblastoma 3D spheroids and an orthotopic xenograft model
Small, 2020, 16 (48), 2004029
Intracerebral injection of graphene oxide nanosheets mitigates microglial activation without inducing acute neurotoxicity: A pilot comparison to other nanomaterials
Cell Reports Physical Science, 2020, 1 (9), 100176
Nose-to-Brain translocation and cerebral biodegradation of thin graphene oxide nanosheets
ACS Nano, 2020, 14 (8), 10168-10186
Splenic capture and in vivo intracellular biodegradation of biological-grade graphene oxide sheets
Advanced Science, 2020, 7, 1903200
Size-dependent pulmonary impact of thin graphene oxide sheets in mice: Towards safe-by-design
Nanoscale Horizons, 2020, 5, 1250-1263
Graphene oxide nanosheets modulate spinal glutamatergic transmission and modify locomotor behaviour in an in vivo zebrafish model
Nanotechnology Characterization Tools for Environment, Health and Safety, Kumar, C.S.S.R. (ed.), Springer, 2019, 1-46
Assessing the adverse effects of two-dimensional materials using cell culture-based models
Advanced Materials Interfaces, 2019, 6, 1900229
Biocompatibility considerations in the design of graphene biomedical materials
FlatChem 2018, 12, 17-25
Graphene-based papers as substrates for cell growth: Characterisation and impact on mammalian cells
Archives of Toxicology, 2018, 92 (11), 3359-3379
Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity
ACS Nano, 2018, 12 (11), 10582-10620
Safety assessment of graphene-based materials: Focus on human health and the environment
2D Materials, 2018, 5, 035020
A blueprint for the synthesis and characterisation of thin graphene oxide with controlled lateral dimensions for biomedicine
ACS Nano, 2018, 12 (2), 1373-1389
Live imaging of label-free graphene oxide reveals critical factors causing oxidative stress-mediated cellular responses
npj 2D Materials and Applications, 2017, 39
Hypochlorite degrades 2D graphene oxide sheets faster than 1D oxidised carbon nanotubes and nanohorns
Nanoscale Horizons, 2017, 2 (5), 284-296
Primary microglia maintain capacity to function despite internalisation and intracellular loading with carbon nanotubes
Nanoscale, 2017, 9 (14), 4642-4645
Direct visualization of carbon nanotube degradation in primary cells by photothermal imaging
Chem, 2017, 2 (3), 322-325
Culture media critically influence graphene oxide effects on plasma membranes
Materials Today, 2017, 20 (1), 1-2
‘Science in the City’: Bringing nanoscale medicine alive
Nanoscale, 2016, 8 (1), 590-601
Intracellular degradation of chemically functionalized carbon nanotubes using a long-term primary microglial culture model
Carbon, 2015, 97, 126-133
Gadolinium-functionalised multi-walled carbon nanotubes as a T1 contrast agent for MRI cell labelling and tracking
ACS Nano, 2015, 9 (8), 7815-7830
Microglia determine brain region-specific neurotoxic responses to chemically functionalized carbon nanotubes
Nanoscale, 2015, 7, 6432-6435
The current graphene safety landscape – a literature mining exercise
ACS Nano, 2015, 9 (2), 1137-1149
Peptide nanofiber complexes with siRNA for deep brain gene silencing by stereotactic neurosurgery
Nanoscale, 2015, 7 (7), 2834-2840
Biodegradation of carbon nanohorns in macrophage cells
Handbook of Safety Assessment of Nanomaterials: From Toxicological Testing to Personalized Medicine, Fadeel, B. (ed.), Pan Stanford Publishing, 2014, 319-340
Biodegradation of Carbon-Based Nanomaterials
Archives of Toxicology, 2014, 89 (9), 1543-1556
The role of p53 in lung macrophages following exposure to a panel of manufactured nanomaterials
Biomaterials, 2014, 35 (29), 8312-8320
Generation of induced pluripotent stem cells from virus-free in vivo reprogramming of BALB/c mouse liver cells
Journal of Visualised Experiments (JoVE), 2013, 82, e50837
In vivo reprogramming of adult somatic cells to pluripotency by overexpression of Yamanaka factors
Advanced Drug Delivery Reviews, 2013, 65 (15), 2061-2062
Carbon nanotubes in medicine & biology — Safety and toxicology (Preface)
Advanced Drug Delivery Reviews, 2013, 65 (15), 2127-2134
Hemotoxicity of carbon nanotubes
Accounts of Chemical Research, 2013, 25 (16), 2258-2268
Safety considerations for graphene: Lessons learnt from carbon nanotubes
Faraday Discussions, 2013, 166, 181-194
Peptide nanofibres as molecular transporters: From self-assembly to in vivo degradation
Journal of Materials Chemistry B, 2013, 1, 4593-4600
Design, engineering and structural integrity of electro-responsive carbon nanotube-poly(methyl) methacrylate hydrogels for pulsatile drug release
Particle and Fibre Toxicology, 2013, 10, 24
Intracellular fate of carbon nanotubes inside murine macrophages: pH-dependent detachment of iron catalyst nanoparticles
PLoS One, 2013, 8 (1), e54754
In vivo cell reprogramming towards pluripotency by virus-free overexpression of defined factors
Particle and Fibre Toxicology, 2012, 9, 46
Critical role of surface chemical modifications induced by length shortening on multi-walled carbon nanotubes-induced toxicity
Nanomedicine, 2012, 7 (10), 1485-1494
In vivo degradation of functionalized carbon nanotubes after stereotactic administration in the brain cortex
Proceedings of the National Academy of Sciences USA, 2011, 108 (27), 10952-10957
Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing
Journal of Materials Chemistry, 2011, 21 (13), 4850-4860
Polyamine functionalized carbon nanotubes: Synthesis, characterization, cytotoxicity and siRNA binding
Particle and Fibre Toxicology, 2011, 8, 3
Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity
HA Schols, RGF Visser and AGF Voragen , editor(s). Pectins and Pectinases. Wageningen Academic Publishers; (2009). p. 313-324.
Health aspects of pectins, PectiCoat: Immobilized enzymatically-tailored pectins to improve the biocompatibility of medical devices
Computer Methods in Biomechanics and Biomedical Engineering (2008). 11, p. 171-172 2 p.
Modulation of fibroblast behaviour by enzymatically-tailored pectins: PectiCoat
J Toxicol Environ Health A (2009) ;72(2):60-73.
Adverse effects of industrial multiwalled carbon nanotubes on human pulmonary cells
Nano Lett (2008), 8(9):2659-63.
Carbon nanotubes in macrophages: imaging and chemical analysis by X-ray fluorescence microscopy
Biochim Biophys Acta (2008);1780(7-8):995-1003
Enzymatically-tailored pectins differentially influence the morphology, adhesion, cell cycle progression and survival of fibroblasts
J Biomed Mater Res A (2008) Sep;86(3):597-606.
Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins
Radiat Prot Dosimetry (2007);127(1-4):86-9.
Heterogeneous accumulation of uranium in the brain of rats
NeuroToxicology (2007). 28, 1, p. 108-113 5 p.
Parental exposure to enriched uranium induced delayed hyperactivity in rat offspring
NeuroToxicology (2006). 27, 2, p. 245-252 7 p.
Chronic ingestion of uranyl nitrate perturbs acetylcholinesterase activity and monoamine metabolism in male rat brain
Toxicology (2005). 212, 2-3, p. 219-226 7 p.
The brain is a target organ after acute exposure to depleted uranium
Neuroscience letters (2005). 390, 1, p. 31-36 5 p.
Bioaccumulation and behavioural effects of depleted uranium in rats exposed to repeated inhalations
Neurotoxicology and Teratology (2005). 27, 6, p. 835-840 5 p.
Changes in sleep-wake cycle after chronic exposure to uranium in rats
Neurotoxicology (2005) ;26(6):1015-20
Enriched but not depleted uranium affects central nervous system in long-term exposed rat
Canadian Journal of Physiology and Pharmacology (2004). 82, 2, p. 161-166 6 p.
Effect of U and (137)Cs chronic contamination on dopamine and serotonin metabolism in the central nervous system of the rat
Professor Kostarelos founded in 2006 and is still acting as the Senior Editor the journal Nanomedicine (Future Medicine, London).
Nanomedicine was the first medicine-oriented journal in the field, addressing the important advances and challenges towards the clinical use of nanoscale-structured materials and devices.
Professor Kostarelos also sits on the Editorial Advisory Board of: