NanoInflammation

The Nanomedicine Lab logo.

The NanoInflammation Team focuses on studying the interactions of nanomaterials with the immune system and associated diseases, using in vitro and in vivo models. We investigate the consequences of nanomaterial interactions with different biological systems (pulmonary, central nervous, cardiovascular, lymphatic, and mononuclear phagocytic systems) at various scales (time, dose, duration) in order to reveal their safety limitations in the context or not of biomedical application.

NanoInflammation Research

Our objectives are not only to identify the physicochemical properties causing adverse outcomes, but also to find the biological mechanisms triggered by the nanomaterial-biological system interaction. By correlation of specific biological responses to the nanomaterial physicochemical properties, and revealing what is safe and what causes harm, the knowledge we are creating helps the production of nanomaterials that are safer-by-design for the patient or the end-user in the intended application.

To address these questions, we use state-of-the-art techniques as well as contribute to the development of new technical modalities to study the nanomaterial-biology interface. We work at all scale levels (from molecules up to full organism), using either specific biomarkers in an adverse outcome pathway approach, or high-content methods to decipher the complexity of the nano/bio interface. We engage with stakeholders such as nanomaterial production industries (chemical or pharmaceutical companies), regulators or standards institutions, in order to make our experimental work as realistic and exposure-driven as possible.

Health impact of engineered nanomaterials

We investigate the impact of (nano)materials on human health in an occupational, end-users or biomedical context. Using different routes of exposure, we study the impact on primary and secondary organs, focusing primarily on the innate and adaptive immune responses. Current investigations are assessing the impact of carbon based materials, 2D materials and micro- /nano- plastics after single or repeated exposure in various in vitro and in vivo models.

Correlative microscopy for toxicological investigations

Using the innate chemical signature of nanomaterials, we perform correlative microscopy to understand the impact of nanomaterials in respect to their spatial location in tissue and cells. In the past we used X ray fluorescence microscopy and light microscopy; in more recent works, we correlated Raman imaging with immunofluorescence imaging. 

TEAM MEMBERS


Dr. Cyril Bussy

Lecturer
NanoInflammation Team Leader


Dr. Luis Visani

Research Associate


Dr. Thomas Loret

Research Associate


Mr. Alex Fordham

PhD Student

NanoInflammation PUBLICATIONS

FILTER

Nanoscale Advances, 2021,3, 4166-4185

Dynamic interactions and intracellular fate of label-free, thin graphene oxide sheets within mammalian cells: role of lateral sheet size

Yingxian Chen, Jack Rivers-Auty, Livia Crică, Katie Barr, Vinicio Rosano, Adrian Arranz, Thomas Loret, Cyrill Bussy, David Spiller, Kostas Kostarelos*, Sandra Vranic*

FULL ABSTRACT

Langmuir, 2021, 37(2): 867–873

Adsorption of P103 nanoaggregates on graphene oxide nanosheets: role of electrostatic forces in improving nanosheet dispersion

Rahul Patil, Debes Ray, Vinod K. Aswal, Cyrill Bussy, Pratap Bahadur, Sanjay Tiwari*

FULL ABSTRACT

Advanced Therapeutics, 2021,4 (1), 2000109

Deep tissue translocation of graphene oxide sheets in human glioblastoma 3D spheroids and an orthotopic xenograft model

Irene de Lázaro, Paul Sharp, Cansu Gurcan, Ahmet Ceylan, Maria Stylianou, Thomas Kisby, Yingxian Chen, Sandra Vranic, Katharine Barr, Hadiseh Taheri, Asuman Ozen, Cyrill Bussy, Acelya Yilmazer*, Kostas Kostarelos*

FULL ABSTRACT

Small, 2020, 16(48): 2004029

Intracerebral injection of graphene oxide nanosheets mitigates microglial activation without inducing acute neurotoxicity: a pilot comparison to other nanomaterials

Corinne Portioli, Cyrill Bussy*, Mariarosa Mazza, Neus Lozano, Dhifaf A. Jasim, Maurizio Prato, Alberto Bianco, Marina Bentivoglio, Kostas Kostarelos*

FULL ABSTRACT

Cell Reports Physical Science, 2020, 1 (9), 100176

Nose-to-Brain translocation and cerebral biodegradation of thin graphene oxide nanosheets

Leon Newman, Artur Filipe Rodrigues, Dhifaf Jasim, Isabella Anna Vacchi, Alberto Bianco, Cyrill Bussy*, Kostas Kostarelos*

FULL ABSTRACT

ACS Nano, 2020, 14 (8), 10168-10186

Splenic capture and in vivo intracellular biodegradation of biological-grade graphene oxide sheets

Leon Newman^, Dhifaf A. Jasim^, Eric Prestat, Neus Lozano, Irene de Lazaro, Yein Nam, Bakri M. Assas, Joanne Pennock, Sarah J. Haigh, Cyrill Bussy*, Kostas Kostarelos*

FULL ABSTRACT

Advanced Science, 2020, 7, 1903200

Size-dependent pulmonary impact of thin graphene oxide sheets in mice: towards safe-by-design

Artur Filipe Rodrigues, Leon Newman, Dhifaf Jasim, Sourav P. Mukherjee, Jun Wang, Isabella Vacchi, Cécilia Ménard-Moyon, Alberto Bianco, Bengt Fadeel*, Kostas Kostarelos*, Cyrill Bussy*

FULL ABSTRACT

Nanoscale Horizons, 2020, 5, 1250

Graphene oxide nanosheets modulate spinal glutamatergic transmission and modify locomotor behaviour in an in vivo zebrafish model

Giada Cellot*, Sandra Vranic, Yu Young Shin, Robyn Worsley, Artur Filipe Rodrigues, Cyrill Bussy, Cinzia Casiraghi, Kostas Kostarelos, Jonathan McDearmid

FULL ABSTRACT

Nanotechnology Characterization Tools for Environment, Health and Safety . Kumar, P. C. (ed.). Springer Nature (2019), p. 1-46

Assessing the adverse effects of two-dimensional materials using cell culture-based models

Lidiane Silva Franqui, Luis Augusto Visani de Luna, Thomas Loret, Diego Stefani Teodoro Martinez, Cyrill Bussy*

FULL ABSTRACT

Advanced Materials Interfaces (2019). 15 p., 1900229.

Biocompatibility considerations in the design of graphene biomedical materials

Christopher J Bullock, Cyrill Bussy

FULL ABSTRACT

FlatChem 2018, 12: 17-25

Graphene-based papers as substrates for cell growth: characterisation and impact on mammalian cells

Dhifaf Jasim, Neus Lozano, Cyrill Bussy, Irina Barbolina, Artur Filipe Rodrigues, Kostya Novoselov, Kostas Kostarelos*

FULL ABSTRACT

Archives of Toxicology, 2018, 92(11): 3359-3379

Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity

Artur Filipe Rodrigues, Leon Newman, Dhifaf A. Jasim, Isabella A. Vacchi, Cécilia Ménard-Moyon, Livia E. Crica, Alberto Bianco, Kostas Kostarelos*, Cyrill Bussy*

FULL ABSTRACT

ACS Nano, 2018, 12 (11): 10582–10620

Safety assessment of graphene-based materials: focus on human health and the environment

Bengt Fadeel,* Cyrill Bussy, Sonia Merino, Ester Vázquez, Emmanuel Flahaut, Florence Mouchet, Lauris Evariste, Laury Gauthier, Joonas Koivisto, Ulla Vogel, Cristina Martín, Lucia G. Delogu, Tina Buerki-Thurnherr, Peter Wick, Didier Beloin-Saint-Pierre, Roland Hischier, Marco Pelin, Fabio Candotto Carniel, Mauro Tretiach, Fabrizia Cesca, Fabio Benfenati, Denis Scaini, Laura Ballerini, Kostas Kostarelos, Maurizio Prato,* Alberto Bianco*

FULL ABSTRACT

2D Materials, 2018, 5: 035020

A blueprint for the synthesis and characterisation of thin graphene oxide with controlled lateral dimensions for biomedicine

Artur Rodrigues, Leon Newman, Neus Lozano, Sourav Mukherjee, Bengt Fadeel, Cyrill Bussy*, Kostas Kostarelos*

FULL ABSTRACT

ACS Nano, 2018, 12(2): 1373-1389

Live imaging of label-free graphene oxide reveals critical factors causing oxidative stress-mediated cellular responses

Sandra Vranic, Artur Rodrigues, Maurizio Buggio, Leon Newman, Michael White, David Spiller, Cyrill Bussy*, Kostas Kostarelos*

FULL ABSTRACT

npj 2D Materials and Applications, 2017, 1 (1): 39

Hypochlorite degrades 2D graphene oxide sheets faster than 1D oxidised carbon nanotubes and nanohorns

Leon Newman, Neus Lozano, Minfang Zhang, Sumio Iijima, Masako Yudasaka, Cyrill Bussy* and Kostas Kostarelos*

FULL ABSTRACT

Nanoscale Horizons, 2017, 2 (5): 284-296

Primary microglia maintain capacity to function despite internalisation and intracellular loading with carbon nanotubes

Cyrill Bussy*, Alberto Bianco, Maurizio Prato, Kostas Kostarelos*

FULL ABSTRACT

Nanoscale, 2017, 9(14): 4642-4645

Direct visualization of carbon nanotube degradation in primary cells by photothermal imaging

Julie Russier, Laura Oudjedi, Martin Piponnier, Cyrill Bussy, Maurizio Prato, Kostas Kostarelos, Brahim Lounis, Alberto Bianco*, Laurent Cognet*

FULL ABSTRACT

Chem, 2017, 2(3): 322–325

Culture media critically influence graphene oxide effects on plasma membranes

Cyrill Bussy* and Kostas Kostarelos*

FULL ABSTRACT

Materials Today, 2017, 20(1): 1–2

‘Science in the City’: Bringing nanoscale medicine alive

Marilena Hadjidemetriou* and Cyrill Bussy*

FULL ABSTRACT

Nanoscale, 2016, 8(1): 590-601

Intracellular degradation of chemically functionalized carbon nanotubes using a long-term primary microglial culture model

Cyrill Bussy, Caroline Hadad, Maurizio Prato, Alberto Bianco, Kostas Kostarelos*

FULL ABSTRACT

Carbon, 2015, 97: 126–133

Gadolinium-functionalised multi-walled carbon nanotubes as a T1 contrast agent for MRI cell labelling and tracking

Ania Servant, Igor Jacobs, Cyrill Bussy, Chiara Fabbro, Tatiana da Ros, Elzbieta Pach, Belen Ballesteros, Maurizio Prato, Klaas Nicolay and Kostas Kostarelos*

FULL ABSTRACT

ACS Nano, 2015, 9(8): 7815-30

Microglia determine brain region-specific neurotoxic responses to chemically functionalized carbon nanotubes

Cyrill Bussy, Khuloud T. Al-Jamal, Jorge Boczkowski, Sophie Lanone, Maurizio Prato, Alberto Bianco and Kostas Kostarelos*

FULL ABSTRACT

Nanoscale, 2015, 7:6432-6435

The current graphene safety landscape – a literature mining exercise

Cyrill Bussy, Dhifaf A. Jasim, Neus Lozano, Daniel Terry, Kostas Kostarelos*

FULL ABSTRACT

ACS Nano, 2015, 9(2):1137-49

Peptide nanofiber complexes with siRNA for deep brain gene silencing by stereotactic neurosurgery

Mariarosa Mazza, Marilena Hadjidemetriou, Irene de Lazaro, Cyrill Bussy, Kostas Kostarelos*

FULL ABSTRACT

Nanoscale, 2015, 7(7):2834-40

Biodegradation of carbon nanohorns in macrophage cells

Minfang Zhang*, Mei Yang, Cyrill Bussy, Sumio Iijima, Kostas Kostarelos, Masako Yudasaka*

FULL ABSTRACT

Handbook of Safety Assessment of Nanomaterials - From Toxicological Testing to Personalized Medicine (Pan Stanford Publishing Pte Ltd), 2014, 319-340

Biodegradation of Carbon-Based Nanomaterials

Cyrill Bussy, Alberto Bianco, Maurizio Prato, Kostas Kostarelos*

FULL ABSTRACT

Archives of toxicology, 2014, 89 (9): 1543-1556

The role of p53 in lung macrophages following exposure to a panel of manufactured nanomaterials

Esther Belade, Sandra Chrusciel, Lucie Armand, Angélique Simon-Deckers, Cyrill Bussy, Philippe Caramelle, Jean-Marie Gagliolo, Laurent Boyer, Sophie Lanone, Jean-Claude Pairon, Ali Kermanizadeh, Jorge Boczkowski

FULL ABSTRACT

Biomaterials, 2014, 35(29): 8312-20

Generation of induced pluripotent stem cells from virus-free in vivo reprogramming of BALB/c mouse liver cells

Irene de Lázaro, Cyrill Bussy, Açelya Yilmazer, Maj Simonsen, Neil Humphreys, Kostas Kostarelos

FULL ABSTRACT

Journal of Visualised Experiments (JoVE), 2013, 82: e50837

In vivo reprogramming of adult somatic cells to pluripotency by overexpression of Yamanaka factors

A.Yilmazer, I.de Lázaro, C.Bussy, K.Kostarelos*

FULL ABSTRACT

Advanced Drug Delivery Reviews, 2013, 65(15): 2061–2062

Carbon nanotubes in medicine & biology — Safety and toxicology (Preface)

C. Bussy*, K. Kostarelos*

FULL ABSTRACT

Advanced Drug Delivery Reviews, 2013, 65(15): 2127–2134

Hemotoxicity of carbon nanotubes

C. Bussy, L. Methven, K. Kostarelos*

FULL ABSTRACT

Faraday Discussions, 2013, 166: 181-194

Peptide nanofibres as molecular transporters: from self-assembly to in vivo degradation

M.Mazza, A.Patel, R.Pons, C.Bussy and K.Kostarelos*

FULL ABSTRACT

Journal of Materials Chemistry B, 2013, 1: 4593-4600

Design, engineering and structural integrity of electro-responsive carbon nanotube-poly(methyl) methacrylate hydrogels for pulsatile drug release

A.Servant, C.Bussy, K.Al-Jamal and K.Kostarelos*

FULL ABSTRACT

Particle and Fibre Toxicology (2013). 10, 1, 24.

Intracellular fate of carbon nanotubes inside murine macrophages: pH-dependent detachment of iron catalyst nanoparticles

Cyrill Bussy, Erwan Paineau, Julien Cambedouzou, Nathalie Brun, Claudie Mory, Barbara Fayard, Murielle Salomé, Mathieu Pinault, Mickaël Huard, Esther Belade, Lucie Armand, Jorge Boczkowski, Pascale Launois, Sophie Lanone

FULL ABSTRACT

PLoS One, 2013, 8(1): e54754

In vivo cell reprogramming towards pluripotency by virus-free overexpression of defined factors

A.Yilmazer, I.de Lázaro, C.Bussy, K.Kostarelos*

FULL ABSTRACT

Particle and Fibre Toxicology (2012). 9, 46.

Critical role of surface chemical modifications induced by length shortening on multi-walled carbon nanotubes-induced toxicity

Cyrill Bussy, Mathieu Pinault, Julien Cambedouzou, Marion Julie Landry, Pascale Jegou, Martine Mayne-l'Hermite, Pascale Launois, Jorge Boczkowski, Sophie Lanone

FULL ABSTRACT

Accounts of Chemical Research, 2013, 25(16): 2258-68

Safety considerations for graphene: lessons learnt from carbon nanotubes

C.Bussy, H.Ali-Boucetta, K.Kostarelos*

FULL ABSTRACT

Nanomedicine, 2012, 7(10): 1485-94

In vivo degradation of functionalized carbon nanotubes after stereotactic administration in the brain cortex

A.Nunes, C.Bussy, L.Gherardini, M.Meneghetti, M.A.Herrero, A.Bianco, M.Prato, T.Pizzorusso, K.T.Al-Jamal and K.Kostarelos*

FULL ABSTRACT

Proceedings of the National Academy of Sciences USA, 2011, 108(27): 10952-10957

Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing

K.T.Al-Jamal, L.Gherardini, G.Bardi, A.Nunes, C.Guo, C.Bussy, M.A.Herrero, A.Bianco, M.Prato, K.Kostarelos*, T.Pizzorusso*

FULL ABSTRACT

Journal of Materials Chemistry, 2011, 21 (13): 4850-4860

Polyamine functionalized carbon nanotubes: synthesis, characterization, cytotoxicity and siRNA binding

P.Singh, Cristian Samorì, F.M.Toma, C.Bussy, A.Nunes, K.T. Al-Jamal, C.Ménard-Moyon, M.Prato*, K.Kostarelos*, A.Bianco*

FULL ABSTRACT

Particle and Fibre Toxicology (2011). 8, 3.

Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity

Lyes Tabet, Cyrill Bussy, Ari Setyan, Angélique Simon-Deckers, Michel J Rossi, Jorge Boczkowski, Sophie Lanone

FULL ABSTRACT

HA Schols, RGF Visser and AGF Voragen , editor(s). Pectins and Pectinases. Wageningen Academic Publishers; (2009). p. 313-324.

Health aspects of pectins, PectiCoat: Immobilized enzymatically-tailored pectins to improve the biocompatibility of medical devices

Muriel Vayssade, Rene Verhoef, A. Haeger, G. Cascardo, Cyrill Bussy, Marlene Gallet, Marco Morra, John Paul Knox, Henk Schols, Marie-Danielle Nagel

FULL ABSTRACT

Computer Methods in Biomechanics and Biomedical Engineering (2008). 11, p. 171-172 2 p.

Modulation of fibroblast behaviour by enzymatically-tailored pectins: PectiCoat

Marie-Danielle Nagel, Pascale Vigneron, Cyrill Bussy, Muriel Vayssade, Jean-Luc Duval, Marlene Gallet, Murielle Dufresne, Rene Verhoef , Marco Morra, JohnPaul Knox, Henk Schols, Giacomo Ceccone , Claudio Della Volpe

FULL ABSTRACT

J Toxicol Environ Health A (2009) ;72(2):60-73.

Adverse effects of industrial multiwalled carbon nanotubes on human pulmonary cells

Lyes Tabet, Cyrill Bussy, Nadia Amara, Ari Setyan, Alain Grodet, Michel J Rossi, Jean-Claude Pairon, Jorge Boczkowski, Sophie Lanone

FULL ABSTRACT

Nano Lett (2008), 8(9):2659-63.

Carbon nanotubes in macrophages: imaging and chemical analysis by X-ray fluorescence microscopy

Cyrill Bussy, Julien Cambedouzou, Sophie Lanone, Emilie Leccia, Vasile Heresanu, Mathieu Pinault, Martine Mayne-l’Hermite, Nathalie Brun, Claudie Mory, Marine Cotte, Jean Doucet, Jorge Boczkowski, Pascale Launois

FULL ABSTRACT

Biochim Biophys Acta (2008);1780(7-8):995-1003

Enzymatically-tailored pectins differentially influence the morphology, adhesion, cell cycle progression and survival of fibroblasts

Marie-Danielle Nagel, René Verhoef, Henk Schols, Marco Morra, J Paul Knox, Giacomo Ceccone, Claudio Della Volpe, Pascale Vigneron, Cyrill Bussy, Marlène Gallet, Elodie Velzenberger, Muriel Vayssade, Giovanna Cascardo, Clara Cassinelli, Ash Haeger, Douglas Gilliland, Ioannis Liakos, Miguel Rodriguez-Valverde, Stefano Siboni

FULL ABSTRACT

J Biomed Mater Res A (2008) Sep;86(3):597-606.

Modulating in vitro bone cell and macrophage behavior by immobilized enzymatically tailored pectins

Cyrill Bussy, René Verhoef, Ash Haeger, Marco Morra, Jean-Luc Duval, Pascale Vigneron, Anne Bensoussan, Elodie Velzenberger, Giovanna Cascardo, Clara Cassinelli, Henk Schols, J Paul Knox, Marie-Danielle Nagel

FULL ABSTRACT

Radiat Prot Dosimetry (2007);127(1-4):86-9.

Heterogeneous accumulation of uranium in the brain of rats

Pascale Houpert, Sandrine Frelon, Marjorie Monleau, Cyrill Bussy, Valérie Chazel, François Paquet

FULL ABSTRACT

NeuroToxicology (2007). 28, 1, p. 108-113 5 p.

Parental exposure to enriched uranium induced delayed hyperactivity in rat offspring

Pascale Houpert, Sandrine Frelon, Philippe Lestaevel, Cyrill Bussy, Patrick Gourmelon, Francois Paquet

FULL ABSTRACT

NeuroToxicology (2006). 27, 2, p. 245-252 7 p.

Chronic ingestion of uranyl nitrate perturbs acetylcholinesterase activity and monoamine metabolism in male rat brain

Cyrill Bussy, Philippe Lestaevel, Bernadette Dhieux, Christine Amourette, Francois Paquet, Patrick Gourmelon, Pascale Houpert

FULL ABSTRACT

Toxicology (2005). 212, 2-3, p. 219-226 7 p.

The brain is a target organ after acute exposure to depleted uranium

P Lestaevel, P Houpert, C Bussy, B Dhieux, P Gourmelon, F Paquet.

FULL ABSTRACT

Neuroscience letters (2005). 390, 1, p. 31-36 5 p.

Bioaccumulation and behavioural effects of depleted uranium in rats exposed to repeated inhalations

Marjorie Monleau, Cyrill Bussy, Philippe Lestaevel, Pascale Houpert, François Paquet, Valérie Chazel

FULL ABSTRACT

Neurotoxicology and Teratology (2005). 27, 6, p. 835-840 5 p.

Changes in sleep-wake cycle after chronic exposure to uranium in rats

Philippe Lestaevel, Cyrill Bussy, François Paquet, Bernadette Dhieux, Didier Clarençon, Pascale Houpert, Patrick Gourmelon

FULL ABSTRACT

Neurotoxicology (2005) ;26(6):1015-20

Enriched but not depleted uranium affects central nervous system in long-term exposed rat

Pascale Houpert, Philippe Lestaevel, Cyrill Bussy, François Paquet, Patrick Gourmelon

FULL ABSTRACT

Canadian Journal of Physiology and Pharmacology (2004). 82, 2, p. 161-166 6 p.

Effect of U and (137)Cs chronic contamination on dopamine and serotonin metabolism in the central nervous system of the rat

P Houpert, P Lestaevel, C Amourette, B Dhieux, C Bussy, and F Paquet

FULL ABSTRACT

COVERS

EDITORIAL

Professor Kostarelos founded in 2006 and is still acting as the Senior Editor the journal Nanomedicine (Future Medicine, London).

Nanomedicine was the first medicine-oriented journal in the field, addressing the important advances and challenges towards the clinical use of nanoscale-structured materials and devices.

Professor Kostarelos also sits on the Editorial Advisory Board of:

Funding

Collaborators

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